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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50723*  Cite 
Description

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

Technical Details

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

6.5_Cell_6_Surface_Rendering is a compressed (zipped) folder containing all files required to render the surface of the bundle. Also included is the ‘ProjectLog’ file produced by Slice and View acquisition software.

This file is a part of Dataset 6, comprised of files related to the Cell #6 of current study. The numbering order of the files reflects the order of operations performed during the analysis. The raw imaging data related to this cell was deposited within Dataset 1 (CIL:50680).

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Inner Ear Outer Hair Cell
Cellular Component
Hair Cell stereocilia
Cuticular plate
Mitochondria
Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)
Biological Context
Biological Process
Native auditory Hair Cells at postnatal day 4
Attribution
Names
Maryna V. Ivanchenko
Marcelo Cicconet
Hoor Al Jandal
Xudong Wu
David P. Corey (http://corey.med.harvard.edu/)
Artur A. Indzhykulian (https://ailab.hms.harvard.edu/)
Link
Scientific Data article
Nature Communications article
OTHER
Massachusetts Eye and Ear; Harvard Medical School
6.5_Cell_6_Surface_Rendering
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50723
Archival Resource Key (ARK)
ark:/b7295/w9cil50723
Grouping This image is part of a group.
Imaging
Image Mode
Serial Electron Microscopy
Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization Methods
Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid)
Immunogold labeling with 10 nm gold beads