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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50766*  Cite 
Description

We show in this study that brain and intestinal stem cells of Drosophila contain a new type of cytoplasmic protrusion which is enriched in mitochondria and that the mitochondria are probably dependent on microtubules for their distribution. The protrusions form mitochondria-enriched cytoplasmic bridges from the stem cells to their niche that we speculate are important in regulating stem cell quiescence and activation.

Technical Details

Specimen preparation: Larval brains (LBs) were prepared from starved (amino acid-restricted) first instar (L1) larvae to enrich for quiescent neural stem cells. The LBs were glutaraldehyde-fixed, tannic acid and OsO4-stained, embedded in resin, thin-sectioned (60-70 nm) and stained with uranyl acetate/lead citrate, then observed by TEM.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
L1 larval brain
Attribution
Names
Sharyn A. Endow
Sara E. Miller
Phuong Thao Ly
Link
Shareable Link
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Duke University EM Imaging Facility
Citation: SA Endow, SE Miller SE & PT Ly 2019 Mitochondria-enriched protrusions are associated with brain and intestinal stem cells in Drosophila. Commun. Biol. Epub 2019 Nov 22 doi: 10.1038/s42003-019-0671-4
D316A_1a_1
BL1
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50766
Archival Resource Key (ARK)
ark:/b7295/w9cil50766
Grouping This image is part of a group.
Imaging
Image Type
Digital
Image Mode
CM12 TEM equipped with AMT digital camera