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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50959*  Cite 
Description

A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding a full therapeutic dose at once at t=0.

Technical Details

populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells.

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Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
RAW 264.7 TIB-71
Cellular Component
whole cell
Biological Context
Biological Process
Cell polarity establishment & maitenance
Attribution
Names
Thomas Buckles
OTHER
W9CILP20451
Individual_Timepoints
Diclofenac
171019
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50956
Archival Resource Key (ARK)
ark:/b7295/w9cil50956
Grouping This image is part of a group.
Imaging
Image Mode
DICM
Visualization Methods
Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective