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CIL:54263*  Cite 

TKL family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.

Technical Details

Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
Cellular Component
Biological Context
Biological Process
Haitao Zhang
Xiaolei Cao
Mei Tang
Guoxuan Zhong
Yuan Si
Haidong Li
Feifeng Zhu
Qinghua Liao
Liuju Li
Jianhui Zhao
Jia Feng
Shuaifeng Li
Chenliang Wang
Manuel Kaulich
Fangwei Wang
Liangyi Chen
Li L
Zongping Xia
Tingbo Liang
Huasong Lu
Xin-Hua Feng
Bin Zhao
Life Sciences Institute Zhejiang University
TKL family
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Visualization Methods
confocal microscopy