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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:54471*  Cite 
Description

Pathology load assessment of de-identified, human, post-mortem Alzheimer's disease and age-matched non-symptomatic donor prefrontal cortex brain tissue. We used these imaging data to inform donor stratification when we generated a large single nuclei RNA sequencing (snRNA-seq) dataset. Given we generated both imaging and snRNA-seq datasets the same location/piece of tissue for each donor, this gives us an unprecedented platform to evaluate cell type/subpopulation transcriptomes and relate them directly to their respective tissue microenvironments.

Technical Details

5 um sections of FFPE prefrontal cortex tissue from post-mortem, human Alzheimer's disease donor labeled for glial fibrillary acidic protein (GFAP), a protein commonly used to identify astrocytes

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
astrocyte
Cellular Component
GFAP - cytoskeletal element
Biological Context
Biological Process
Alzheimer's disease patient
Attribution
Names
Jessica S. Sadick
Shane A. Liddelow
OTHER
central nervous system
brain
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL54471
Archival Resource Key (ARK)
ark:/b7295/w9cil54471
Grouping This image is part of a group.
Imaging
Image Mode
bright-field microscopy
Visualization Methods
GFAP IHC using HRP/DAB