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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7240*  Cite 
Description

HeLa cell was fixed by MeOH and stained with microtubules (green) and central spindlin protein MKLP1 (red). MKLP1 (mitotic kinesin-like protein) was well aligned on midzone microtubules during cytokinesis. Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against MLKP1 and FITC labeled DM1a antibody against microtubules. Secondary antibody was Alexa 594 for MLKP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected wti an OrCA ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
microtubule
Biological Context
Biological Process
microtubule-based movement
Attribution
Names
Hu, Chi-Kuo
OTHER
Tim Mitchison Lab
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7240
Archival Resource Key (ARK)
ark:/b7295/w9cil7240
Grouping This image is part of a group.
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.064µm
Y 1024px 0.064µm
Channel Wavelength
1 488nm
2 568nm