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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7915*  Cite 
Description

Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, and 47 images at 5 sec intervals.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
MC:BALB/c 3T3 fibroblast
Cellular Component
nuclear chromatin
Biological Context
Molecular Function
mobility of nuclear protein
Attribution
Names
Ghosh, Rajarshi
Woodcock, Chris
Pubmed
20679481
Pubmed
11130729
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7915
Archival Resource Key (ARK)
ark:/b7295/w9cil7915
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
quantity of GFP-protein
Visualization Methods
Green fluorescent proteins from Anthozoa
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.05µm
Y 512px 0.05µm
Time 5 seconds 50