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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:831*  Cite 

Rabbit skeletal muscle fibers were minced in a ATP-containing relaxing buffer and dissociated into thick and thin filaments by mild blending. The isolated filaments were then deposited onto mica flakes, quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operating at 100 kev. Images were recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED. All the filaments seen in this micrograph are thin, actin-containing filaments. Note the striped appearance representing a helical rise due to subunit packing of g-actin monomers. A few hemocyanin molecules were included in this preparation for size calibration. RIGHT image of a stereo pair.

Biological Sources
NCBI Organism Classification
Oryctolagus cuniculus
Cell Type
skeletal muscle cell
Cellular Component
Biological Context
Biological Process
skeletal muscle contraction
Molecular Function
structural constituent of muscle
Ip, Wallace
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
unfixed tissue
Relation To Intact Cell
isolated subcellular component
Spatial Axis Image Size Pixel Size
X 4163px 0.00026µm
Y 2790px 0.00026µm