Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9069*  Cite 
Description

Saos2 cell line immunostained with Tom20 (mitochondrial outer membrane protein, green) and DLP1 (dynamin like protein, red) antibody. Cell population synchronized at G1-S transition of the cell cycle. This Saos2 cell line was serum starved (0.1%) for 3 days and released in FCCP (10 mM) ) for 16 hours. The image is a single 3 micron confocal section collected on a Zeiss LSM 510 with the following parameters: Green: Ex: 488 nm laser, Em: Band Pass 500-550nm, Red: 543 nm laser, Em: Long pass 560 nm.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
osteosarcoma
Cell Line
Saos-2
Cellular Component
mitochondrial outer membrane
dynamin like protein
Biological Context
Biological Process
G1/S transition of mitotic cell cycle
Attribution
Names
Kasturi Mitra
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9069
Archival Resource Key (ARK)
ark:/b7295/w9cil9069
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
color combine
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.29µm
Y 512px 0.29µm
Channel Wavelength
1 488, 543nm