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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9098*  Cite 
Description

Hela cells transiently transfected with EGFP-GL-GPI show the organization of the membrane thru the distribution of GPI anchored protein. This image is the maximum z projection image that accompanies the related z stack. The images were collected on a Zeiss spinning disk confocal microscope using a 63 X 1.4 NA oil immersion objective. 56 z slices at 0.27 micron intervals were collected with a 450 ms exposure using a 488 ht.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
permanent cell line cell
epithelial cell
Cell Line
HeLa
Cellular Component
anchored to plasma membrane
Biological Context
Biological Process
cellular membrane organization
Molecular Function
GPI anchor binding
Attribution
Names
Prabuddha Sengupta
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9098
Archival Resource Key (ARK)
ark:/b7295/w9cil9098
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.11µm
Y 512px 0.11µm
Z —— 0.27µm