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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9928*  Cite 
Description

A high resolution tangential view of the surface of a non-dividing cell shows several ribbons of microtubules between the alveolar sac and the epiplasm. The thick fibrous layer associated with the epiplasm is evident, and the layer of mitochondria under the epiplasm is wrapped with rough ER. A peroxisome lies nearby and mucocysts and toxicysts are evident. TEM taken on 2/18/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
cell cortex
cortical microtubule cytoskeleton
extrusomes
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9928
Archival Resource Key (ARK)
ark:/b7295/w9cil9928
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4696px 1nm
Y 3486px 1nm