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Reconstruction
Display image description
Single computed slice through a tomographic reconstruction of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This reconstruction is the 22nd in a series of 26 serial reconstructions through the cell soma. The complete reconstruction can be viewed under MP7503.
Full resolution image description
File contains TxBR generated tomographic reconstructions
Downsampled image description
File contains binned down TxBR generated tomographic reconstructions binned by a factor of 8. Note that stated resolution is for full resolution volume and not for the downsampled volume.
File format
MRC
Volume_dimension
7600, 7600, 384
Volume scale
0.00153, 0.00153, 0.00153
Animation description
Animation through the computed slices of a tomographic reconstruction of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This reconstruction is the 22nd in a series of 26 serial reconstructions through the cell soma. The complete reconstruction can be viewed under MP7503.

Image 2D
Display image description
Electron micrograph of the soma of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy at zero degrees tilt. This section is the 22nd in a series of 26 serial semi-thick sections through the astrocyte soma used for a serial tomographic reconstruction (MP7503).
Full resolution image description
File contains all IMOD and TxBR files (st, fid, preali, *com, *log, *mat, etc...)
Animation description
Animated tilt series of the soma of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This section is the 22nd in a series of 26 serial semi-thick sections through the astrocyte soma used for a serial tomographic reconstruction (MP7503).

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:6736*
Project: P2023
Project name
Whole Brain Catalog Project
Description
The Whole Brain Catalog™ is a ground-breaking, open-source, 3-D virtual environment developed by a team of researchers from UC San Diego under the Whole Brain Project™. The Catalog aims to connect members of the international neuroscience community to facilitate solutions for today's intractable challenges in brain research through cooperation and crowd sourcing. CCDB provides the backend services for very large scale image data sets on mouse brain derived from high resolution light and electron microscopy
Funding agency
Ted Waitt Family Foundation
Leader(s)
Mark Ellisman
Stephen Larson
Collaborator(s)
Sarah Maynard
Eric Bushong
Maryann Martone
Start date
08-05-2009
End date
unspecified
 
Experiment
Experiment ID
6395
Experiment date
10-01-2009
Title
Ultrastructural analysis of cerebellar and hippocampal neuropil
Purpose
To use serial electron tomography to determine differences in the ultrastructure of neuropil between the cerebellum and the hippocampus. Samples were prepared using conventional EM protocol.
Experimenter(s)
Eric Bushong
Monica Berlanga
Microscopy product
Microscopy product ID
6736
Instrument
JEOL 4000 #2
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
astrosoma-g4s5
Spatial Axis Image Size Pixel Size
X 7600px
Y 7600px
Subject
Species
mouse
Scientific name
Mus musculus
Strain
C57BL/6NHsd
Group by
conventional EM
Treatment
Strong aldehyde and osmium fixation, en bloc uranyl acetate, standard dehydration, and epoxy embedding using a normal male, C57 black 1-month-old mouse.
Age
1 months
Age class
young adult
Tissue section
Anatomical location
hippocampus, from CA1 stratum radiatum
Microtome
ultramicrotome
Tissue product storage
embedded
Thickness
0.5 µm
Specimen description
Organ
brain
System
central nervous system
Structure
soma
Cell type
protoplasmic astrocyte
Imaging parameters
Type
Electron microscopy product
Recording medium
8K CCD
Magification
5000
Accelerating voltage
400 keV
Specimen preparation
Protocol used
Perfuse animal with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4, at 35 degrees Celsius for 10 minutes. Remove tissue and fix on ice for 1-2 hours. During this time the tissue is further dissected.Wash 5x5 minutes in cold 0.15M sodium cacodylate buffer.Postfix in 1% OsO4 in 0.15M sodium cacodylate buffer on ice for 1 hour.Rinse in cold ddH2O 4x5 minutes.Place tissue into 2% uranyl acetate in ddH2O on ice for 2 hours.Rinse tissue in ice cold ddH2O 3x5 minutes.Dehydrate tissue in ethanol series 20, 50, 70, 90, 100% for 5 minutes each on ice.Complete dehydration with 100% acetone 2x10 minutes, first on ice, second at room temperature.Infiltrate with solution of 50% acetone and 50% durcupan ACM resin overnight in scintillation vials with caps on.Transfer tissue to foil dishes and change to 100% resin for 1 hour. Place into fresh resin for an additional 2 hours.Place in fresh resin and flat embed using 2 mould release treated slides. Polymerize in 60 degree Celsius vacuum oven for 24-48 hours.
Imaging product type
Type
Serial section
Description
26 section series used for serial tomographic reconstruction
Z resolution
0.5 µm
Notes
Section 22 of 26
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees