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Reconstruction
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Double tilt electron tomographic reconstruction through the neuropil of the stratum radiatum of hippocampal area CA1 in the adult rat. Preparation was stained with Zn acetate and embedded in Spurr's resin.
Full resolution image description
Volume file in tiff format (1e5.tif) of the neuropil of the stratum radiatum of hippocampal area CA1 in the adult rat.
File format
TIFF
Volume_dimension
2241, 2242, 85
Animation description
Animation through the computed slices of a double tilt electron tomographic reconstruction through the neuropil of the stratum radiatum of hippocampal area CA1 in the adult rat. Preparation was stained with Zn acetate and embedded in Spurr's resin.
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Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:8641*  Cite 
Project: P1194
Project name
Electron tomographic analysis of synaptic structure in the adult rat cortex
Description
Novel specimen preparation techniques were combined with electron tomography to provide new views of synaptic structure in the cortex of the adult rat
Leader(s)
Alain C. Burette
Collaborator(s)
Richard Weinberg
John Crum
Mark Ellisman
Maryann Martone
Start date
09-01-2000
End date
09-01-2000
 
Experiment
Experiment ID
8475
Title
Tomographic imaging of synapses
Purpose
Imaging of synapses prepared with an osmium free aldehyde fixation protocol
Experimenter(s)
Alain C. Burette
Microscopy product
Microscopy product ID
8641
Instrument
JEOL4000EX
Microscopy type
IVEM
Product type
DOUBLE TILT
Image basename
1e5
Subject
Species
rat
Scientific name
rattus rattus
Strain
Sprague Dawley
Age class
adult
Tissue section
Anatomical location
Hippocampal area CA1 or cortical area S1
Thickness
0.12 µm
Specimen description
Organ
brain
System
central nervous system
Structure
neuropil
Imaging parameters
Type
Electron microscopy product
Magification
0
Imaging product type
Type
Double tilt
X min range
-70 degrees
X max range
70 degrees
X tilt increment
2 degrees
Y min range
-70 degrees
Y max range
70 degrees
Y tilt increment
2 degrees