Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (this image) and anaphase cells (CIL#13886). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-CNM67–TEM1 from a CEN plasmid...

Constitutive targeting of Tem1 to the spindle pole body (SPB) in metaphase cells (CIL# 13884) and anaphase cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-BFA1–TEM1 from a CEN plasmid...

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged MAD2. MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the o...

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged MAD2. MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the o...

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly ...

Intracellular localization of GFP-GOLPH3 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutan...

Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vp...

Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOL...

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta mutant cells arrested in S phase. Movie plays at 100x real time and is movie S6 in Proc Natl Acad Sci. 2009. 10...

3HA-Bfa1 (red) is now symmetrically localized to both spindle pole bodies (SPB) in cells with constitutive targeting of Tem1 to both SPBs in metaphase as determined by spindle morphology (tubulin, gre...