Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of pha...

Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of pha...

Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of pha...

Most images show fractures through the outer membrane of mitochondria and SR/T tubules of dyads nestled in long grooves of the mitochondrial surface. All leaflets of the membranes appear in the image...

Quick-freeze deep-etch images show a more three dimensional view of acidosomes surrounding a phagosome. Acidosomes are unique in that they contain indentations of their membrane. The lumen has a honey...

A high magnification view of the intimate docking of acidosomes with the phagosome is particularly apparent where the P-face of the acidosome lies against the E-face of the phagosome. Numerous large I...

Higher magnification of a QF-DE image of an acidosome with a large inward indentation of its membrane. The fractured P-face of the indentation shows many large IMPs. The acidosome lumen is honeycombed...

The cytopharynx/nascent digestive vacuole (phagosome) has an E-fracture face containing a high number of IMPs. Interestingly these IMPs are sometimes organized into plaques where there are several sho...

In addition to plates the plasma membrane contains IMPs on its P-fracture face in ring or parenthesis shape at sites of trichocyst secretion. A ring actually consists of two circles of IMPs and signif...

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were exposed to low salt buffer, cryofixed, freeze-fractured, and surface replicas observed by transmiss...