DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with galactose. Overexpression of Kin4 arrests cells in anaphase. Compare with control image CIL# 13895. Image is Fig...

DIC image of cells expressing Kin4 from the GAL1-10 promoter and growing in medium with glucose. This is the control image for CIL# 13894 in which overexpression of Kin4 arrests cells in anaphase. Ima...

DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with glucose. This is the control image f...

DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of T...

Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #1387...

Tem1 (green) localizes to spindle pole bodies (SPB) during metaphase. Control image for CIL# 13876 in which Tem1 loading onto SPBs was severely affected in bfa1∆ cells. DAPI (blue) and DIC (hidden) ...

Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytopl...

Tem1 (green) normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase; however, Tem1's ability to localize to the SPB is severely decreased in bfa...

Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a misaligned spindle, CIL ...

Tem1 (green) primarily localizes to the daughter cell spindle pole body (SPB) during anaphase. Control image for CIL# 13870 in which Tem1 loading onto SPBs was severely affected in bfa1Δ cells. DAPI ...