Wild-type PINK1-YFP (green) is rapidly degraded in HeLa cells. HeLa cells expressing PINK1-YFP were stained with 10nM MitoTracker Red (red) for 30 min to see mitochondrial morphology and then treated ...

Expression of R98F PINK1-YFP (green) induces mitochondrial translocation of mCherry-Parkin (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone...

YFP-Parkin (green) does not translocate to mitochondria (red) in HeLa cells. HeLa cells stably expressing YFP-Parkin were treated with DMSO for 3 hrs and then stained with TMRE, a cationic fluorescent...

YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are treated with the proteosome inhibitor, MG132. HeLa cells stably expressing YFP-Parkin were treated with MG132 (10µM) ...

Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are perme...

YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA f...

PINK1-YFP R98F (green) is localized to mitochondria in the absence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). The R98F mutation in PINK1-YFP partially in...

Expression of transmembrane domain deleted-PINK1-YFP (green) is stabilized in the mitochondira, but does not induce mitochondrial translocation of mCherry-Parkin (red) in PINK1 knock-out mouse embryon...

Mitochondria in HeLa cells are identified by mito-YFP (green). When fixed cells are unpermeabilized (not treated with detergent), antibodies to cyctochrome c (red), a mitochondrial matrix marker, and ...

YFP-WT PINK1 (green) shows mitochondrial localization in HeLa cells following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are...